quantitative chemical biology of RNA structure and recognition
RNA molecules do not act as simple linear messages. They fold into dynamic structures that control how genes are expressed by shaping interactions with proteins, enzymes, small molecules, and therapeutic oligonucleotides. Our lab uses quantitative chemical biology to measure how RNA folds and behaves in cells, to uncover the mechanisms that govern RNA recognition and regulation, and to build predictive frameworks for modulating these interactions. By connecting RNA structure to function, we aim to answer foundational questions in gene regulation while advancing new strategies for cancer biology, RNA therapeutics, and antibiotic discovery.
Measuring RNA structure in living systems
We develop and apply chemical probing approaches to determine how RNA folds in vitro and in cells. These experiments let us move beyond static pictures of RNA to ask how cellular context changes structure, accessibility, and conformational behavior. By measuring RNA structure directly in biological environments, we can identify the features that are most relevant for regulation and targeting.
Defining the rules of RNA recognition
RNA function depends on selective interactions with proteins, enzymes, small molecules, and other ligands. We use designed RNA libraries and high-throughput measurements to dissect how sequence, structure, and local context control binding, catalysis, and molecular specificity. Our goal is to build mechanistic and quantitative models that explain why some RNA interactions occur efficiently and selectively while others do not.
Predicting and engineering RNA behavior
A major goal of the lab is to move from description to prediction. We combine sequencing-based measurements, quantitative analysis, and experimentally controlled RNA designs to connect structural changes to measurable biological outcomes. This work helps establish the principles needed to predict how perturbing RNA sequence or structure will alter interaction strength, regulatory activity, and cellular function.
Targeting RNA in disease
We are motivated by both foundational questions and translational opportunities. Insights from our mechanistic studies are applied to problems in cancer and infectious disease, where RNA structure can create opportunities for therapeutic intervention. By understanding how RNA folds and how it is recognized in cells, we aim to support the development of RNA-targeted strategies including antisense approaches, small-molecule discovery, and new concepts for antibiotic design.